Detection of Functional Matrix Metalloproteinases by Zymography
نویسندگان
چکیده
منابع مشابه
Detection of Functional Matrix Metalloproteinases by Zymography
Matrix metalloproteinases (MMPs) are zinc-containing endopeptidases. They degrade proteins by cleavage of peptide bonds. More than twenty MMPs have been identified and are separated into six groups based on their structure and substrate specificity (collagenases, gelatinases, membrane type [MT-MMP], stromelysins, matrilysins, and others). MMPs play a critical role in cell invasion, cartilage de...
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References 1. Tanumihardjo SA, Cheng JC, Permaesih D, Muherdiyantiningsih, Rustan E, Muhilal, et al. Refinement of the modified-relative-dose-response test as a method for assessing vitamin A status in a field setting: experience with Indonesian children. Am J Clin Nutr 1996;64: 966–71. 2. de Pee S, Yuniar Y, West CE, Muhilal. Evaluation of biochemical indicators of vitamin A status in breast-f...
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a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...
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متن کاملMatrix metalloproteinases
What makes an MMP an MMP? They all have a conserved zinc-binding site in their catalytic domain (HEXGHXXGXXH), and the sequence PRCGVPD in the propeptide domain. The cysteine residue serves to anchor the propeptide to the catalytic zinc, suppressing enzymatic activity in a manner that is not unlike a thumb in an open mouth. Release of this interaction results in proteolytic activity. MMPs are a...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2010
ISSN: 1940-087X
DOI: 10.3791/2445